DTI JadeAmp FabTaq Premix

DTI JadeAmp FabTaq Premix PCR Master Mix is a loading-dye-added version of JadeAmp FabTaq MAX PCR Master Mix that is optimized for great performance and convenience in both standard and high-throughput PCR applications. This master mix includes an optimized buffer, PCR enzyme, dNTP mixture, gel loading dye (green), and a density reagent in a 2X premix format. Simply add primers and DNA template. Following PCR, amplicons can be used directly in several ways.

PCR tube contents can be loaded directly onto an agarose gel for electrophoresis or used directly in downstream applications such as restriction enzyme digestion, TA cloning, and direct sequencing. DTI JadeAmp FabTaq Premix PCR Master Mix can be used to amplify genomic targets up to ~5 kb and is compatible with GC- and AT-rich targets.

When 5 µl of the loading-dye-added PCR master mix is used for electrophoresis on an 1% Agarose gel, the blue dye front is detected around 3–5 kb, and the yellow dye is detected below 50 bp. These dyes have absorptions at approximately 260 nm and 420 nm, respectively. The dyes can be removed by gel purification or PCR cleanup using the NucleoSpin Gel and PCR Clean-Up kit (where available), if necessary.

Application

DTI JadeAmp premixes outperforms Competitor(C) Red mix for GC-rich targets Results Amplification of GC-rich targets Our results showed that the non-hot-start DTI JadeAmp FabTaq Premix and DTI JadeAmp Max HiFid Taq Premix gave minimal background amplification with GC-rich targets. Non-hot-start Competitor(C) Red Mix, on the other hand, resulted in nonspecific amplification and was unable to amplify the target with a high GC content (72.3%). While DTI JadeAmp FabTaq Premix failed to amplify the TGFB-1 gene that is 4 kb long with a 63.1% GC content, DTI JadeAmp Max HiFid Taq Premix was able to generate a clean and distinct band. We were unable to amplify the 4-kb TGFB-1 target using Competitor(C) Red Mix. Conclusions DTI JadeAmp FabTaq Premix and DTI JadeAmp Max HiFid Taq Premix (non-hot-start and hot-start) outperformed Competitor(C) Red Mix in background amplification, target GC-content, and target length. The DTI JadeAmp series gave minimal background amplification and better performance on GC-rich targets. Additionally, the DTI JadeAmp Max HiFid Taq Premix master mixes were able to amplify long targets while Competitor(C) Red Mix failed to amplify the 4-kb target gene TGFB-1. With DTI's JadeAmp series of PCR master mixes, you can streamline your genotyping workflow without worrying about GC content of your target or nonspecific background amplification.