Cloning

Cloning is the process of generating a genetically identical copy of a cell or an organism or duplication of any kind of biological material such as a piece of DNA or an individual cell. And molecular cloning is a technique that enables scientists to isolate specific DNA sequences and generate multiple identical copies by inserting them into host organisms for replication.
Molecular cloning techniques are diverse, with new methods being continually developed. It is no longer limited to restriction enzymes, there are many technologies, and the challenge is to figure out what system works best for your applications.

Some important factors to consider

Speed
Cost
Difficulty
Efficiency
The Number of Inserts

Types of Molecular Cloning

traditional cloning

ta cloning

seamless cloning

Insert Preparation

Starting Material
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OR
OR
Annealed Oligos
OR
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Cloning Techniques
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Estimated Total Time
1 hr. 20 min. - 2 hr. 40 min.
2 hr. – 2 hr. 30 min
2 hr. 15 min.
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Transformation

DNA Isolation

(Plasmid Purification)

DNA Analysis

Protein Expression

Functional Analysis

Site-Directed Mutagenesis

Vector Preparation

Starting Material
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Cloning Techniques
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Restriction Enzyme (RE) Digestion

PCR

Estimated Total Time
20 min. - 2 hr., 25 min.
3 hr., 45 min