Cloning
Cloning is the process of generating a genetically identical copy of a cell or an organism or duplication of any kind of biological material such as a piece of DNA or an individual cell. And molecular cloning is a technique that enables scientists to isolate specific DNA sequences and generate multiple identical copies by inserting them into host organisms for replication.
Molecular cloning techniques are diverse, with new methods being continually developed. It is no longer limited to restriction enzymes, there are many technologies, and the challenge is to figure out what system works best for your applications.
Some important factors to consider
 Speed |  Cost |  Difficulty |  Efficiency |  The Number of Inserts |
Types of Molecular Cloning
traditional cloning
ta cloning
seamless cloning
Insert Preparation
Starting Material
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| OR | OR | OR |  Annealed Oligos OR |
Cloning Techniques
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Estimated Total Time
1 hr. 20 min. - 2 hr. 40 min.
2 hr. – 2 hr. 30 min
2 hr. 15 min.
Transformation
DNA Isolation
(Plasmid Purification)
DNA Analysis
Protein Expression
Functional Analysis
Site-Directed Mutagenesis
Vector Preparation
Starting Material
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Cloning Techniques
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Restriction Enzyme (RE) Digestion
PCR
Estimated Total Time
20 min. - 2 hr., 25 min.
3 hr., 45 min