Cloning
Cloning is the process of generating a genetically identical copy of a cell or an organism or duplication of any kind of biological material such as a piece of DNA or an individual cell. And molecular cloning is a technique that enables scientists to isolate specific DNA sequences and generate multiple identical copies by inserting them into host organisms for replication.
Molecular cloning techniques are diverse, with new methods being continually developed. It is no longer limited to restriction enzymes, there are many technologies, and the challenge is to figure out what system works best for your applications.
Some important factors to consider
 Speed |  Cost |  Difficulty |  Efficiency |  The Number of Inserts |
Types of Molecular Cloning
traditional cloning
ta cloning
seamless cloning
Insert Preparation
Starting Material
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 Plasmid OR |  gDNA OR |  PCR Products OR |  Annealed Oligos OR |  cDNA RNA cDNA Synthesis |
Traditional Cloning
(RE Digestion & Ligation)
PCR Cloning
(TA & Blunt-End)
Seamless Cloning
(Gene Assembly)
Estimated Total Time*
1 hr. 20 min. - 2 hr. 40 min.
2 hr. – 2 hr. 30 min
2 hr. 15 min.