DTI Bca DNA Polymerase - Glycerol Free (8U/μl)
DTI Bca DNA Polymerase - Glycerol Free (8U/μl)
DTI Bca DNA Polymerase- Glycerol Free originates from a thermophilic bacterium Bacillus caldotenax, that lacks 5’ → 3’ exonuclease activity. By introducing mutations into the protein, the activity of both DNA polymerase and reverse transcriptase is highly enhanced while exerting the original strong strand-displacement activity. Therefore, the isothermal amplifications using this enzyme enable to amplify the target product from a little nucleic acid in a shorter time. This enzyme is originally isolated and developed independently by Takara Bio Inc, Japan. DTI manufactures and sells it under an agreement with Takara Bio Inc.
SKU:DT0104.80
DTI Bca DNA Polymerase- Glycerol Free (8U/μl)
| Brand | Catalogue Number | Pack Size |
|---|---|---|
| DTI Bca DNA Polymerase- Glycerol Free (8U/μl) | DT0104.80 | 80 U |
- Overview:
- Improved sensitivity compared to BcaBEST DNA Polymerase
- Reaction completes in 30 min or less
- High thermostability
- Compatible with real-time fluorescent monitoring and colorimetric/visual detection methods
- Storage and Shipment Conditions: -80°C
- Components:
Components Pack Size DTI Bca DNA Polymerase- Glycerol Free (8U/μl) 10 µl * Customized pack size available - Materials required but not provided:
- 2X Bca BEST buffer (Component of Takara Cat# RR380A)
- 10X target specific LAMP primer mix
- Template DNA/cDNA
- For RT-LAMP, Reverse Transcriptase
- Nuclease free water
- For real time LAMP assay: Inter-calating dye, Thermal cycler
- Source: Escherichia coli carrying a plasmid containing the gene for BCA DNA Polymerase
- Unit definition: One unit is defined as the amount of enzyme that incorporates 210 nmol of dNTP into acid-insoluble precipitate in 30 minute at 65°C.
- Application:
- LAMP (Loop-Mediated Isothermal Amplification)
- RCA (Rolling Circle Amplification)
- Precautions for use:
- Don’t mix the enzyme vigorously.
- Extreme cautions should be taken during reaction preparation to avoid contamination. If analyzing the products after reaction, execute in a different area from the reaction preparation.
- Reaction temperatures above 70℃ are not recommended. Hence this enzyme can’t be used for conventional PCR.
DTI Bca DNA Polymerase - Glycerol Free - Protocol and Downloads
| Document | Download Link |
|---|---|
| DTI Bca DNA Polymerase - Glycerol Free - Protocol | Download User Manual |
| DTI Bca DNA Polymerase - Glycerol Free - Certificate of Analysis | Download CoA - DT0104.80 |
| DTI Bca DNA Polymerase - Glycerol Free - Promotional Document | Download Flyer |
Results
Experimental Sample
1. LAMP assay: Target gene was detected using 0.01 – 0.01 ng of E. coli genomic DNA when the isothermal amplification was performed using the enzyme. The protocol used was: Incubate at 63℃ for 90 minutes.
Figure 1: LAMP assay showing target gene detection using E. coli genomic DNA.
2. RT-LAMP assay: Target gene was detected using 0.01 – 0.01 ng of human total RNA when the RT-isothermal amplification was performed using AMV reverse transcriptase (Takara cat# 2630A) and Bca DNA polymerase. The protocol used was: Incubate at 63℃ for 90 minutes.
Figure 2: RT-LAMP assay showing target gene detection using human total RNA.