Takara Taq™ Hot Start Version
Takara Taq™ Hot Start Version
The hot-start versions of Takara Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody that binds to Taq polymerase, thereby preventing DNA synthesis at room temperature. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot-start PCR enzyme prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during PCR assembly.
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Takara Taq™ Hot Start Version
| Document | Download Link |
|---|---|
Takara Taq™ Hot Start Version |
Download User Manual |
Takara Taq™ Hot Start Version |
Download COA |
Takara Taq™ Hot Start Version
- Hot-start PCR
- Multiplex PCR
Takara Taq™ Hot Start Version
- Takara Taq DNA Polymerase Hot Start Version
Enzyme, buffer, and dNTPs are supplied as separate components. - Premix Taq DNA Polymerase Hot Start Version
2X premix containing Takara Taq HS enzyme, dNTPs, and an optimized reaction buffer. - Takara Taq HS Perfect Mix
2X hot-start premix optimized for fast reactions. It contains a modified Taq DNA polymerase, which lacks exonuclease activities, and is compatible with a 20 sec/kb extension step.
Takara Taq™ Hot Start Version
- If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Takara Taq™ Hot Start Version
- Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.