TaKaRa Ex Taq® Hot Start Version (250 U)

The hot-start versions of TaKaRa Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody that binds to Taq polymerase, thereby preventing DNA synthesis at room temperature. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot-start PCR enzyme prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during PCR assembly.

Several hot-start versions of Takara Taq are available:

TaKaRa Taq DNA Polymerase Hot Start Version—enzyme, buffer, and dNTPs are supplied as separate components
Premix Taq DNA Polymerase Hot Start Version—2X premix containing Takara Taq HS enzyme, dNTPs, and an optimized reaction buffer
TaKaRa Taq HS Perfect Mix—2X hot-start premix optimized for fast reactions. It contains a modified Taq DNA polymerase, which lacks exonuclease activities, and is compatible with a 20 sec/kb extension step

Application

-Ideal for high-fidelity, high-yield endpoint PCR
-Amplifications requiring room-temperature reaction assembly

Citations

Association of maternal genetics with the gut microbiome and eucalypt diet selection in captive koalas Kotaro Kondo, Mirei Suzuki, ..., Anindita Bhadra PeerJ | 2024 May 27 | Read Article ".. samples using QIAamp Fast DNA Stool Mini Kit (Qiagen GmbH).. Next, using TaKaRa Ex Taq Hot Start Version (Takara Bio Inc., Shiga, Japan), the mitochondrial DNA control region (D-loop) was amplified via polymerase chain reaction (PCR) using the following primers: MaL15999M (ACC ATC AAC ACC CAA AGC TGA) and MaH16498M (CCT GAA GTA GCA ACC AGT AG) (Fumagalli et al. 1997).. The PCR conditions were as follows: initial denaturation (94 °C .." More...