TaKaRa Bradford Protein Assay Kit can quickly measure protein concentration within a range of 1 - 1,000 μg/ml using a simple protocol. The Bradford assay is based on measurement of the absorbance shift from 465 nm to 595 nm (brown to blue) that occurs upon Coomassie dye binding with protein. This change is proportionate to the amount of protein in solution, making it possible to assay protein concentration by measuring absorbance at 595 nm. The protein/dye complex is stable for 5 to 60 minutes after the start of the reaction. It is possible to perform measurement with this kit even in the presence of a reducing agents, but surfactants in the protein solution may result in inaccurate measurements. Additionally, coloration differs greatly depending on the type of protein.When the standard protocol is followed, this kit contains sufficient reagents for 500 assays when a 1 ml reaction volume is used and 2,500 assays when a 200 μl reaction volume is used. When thelow-concentration protocol is used, the kit can perform 1,000 assays with a 1 ml reaction volume and 5,000 assays with a 200 μl reaction volume.
