This product has no images.


RNAiso Blood is an RNA extraction reagent that can quickly isolate total RNA from liquid samples, including blood and high-water content plant samples. This reagent allows for a simple protocol; after cell lysis in RNAiso Blood solution, chloroform is added to the homogenate, the solution is mixed well and centrifuged to separate the mixture into 3 layers. The aqueous layer on top contains the RNA, the semi-solid middle layer contains DNA, and the bottom organic layer (red) contains pro­teins, polysaccharides, fatty acids, cell debris, and a small amount of DNA. Total RNA can be recov­ered from the top layer by performing isopropanol precipitation. Using RNAiso Blood, total RNA extraction can be completed in approximately 1 hour. Because the resulting total RNA will be almost completely free of DNA and protein, it can be used directly in downstream applications including RT-PCR, Northern blot analysis, mRNA isolation, and in vitro translation reactions

Catalogue No:
Price
sjdk
Rs.
6500.00
 
Rs.
Product OverView
expand_less
expand_more

RNAiso Blood is an RNA extraction reagent that can quickly isolate total RNA from liquid samples, including blood and high-water content plant samples. This reagent allows for a simple protocol; after cell lysis in RNAiso Blood solution, chloroform is added to the homogenate, the solution is mixed well and centrifuged to separate the mixture into 3 layers. The aqueous layer on top contains the RNA, the semi-solid middle layer contains DNA, and the bottom organic layer (red) contains pro­teins, polysaccharides, fatty acids, cell debris, and a small amount of DNA. Total RNA can be recov­ered from the top layer by performing isopropanol precipitation. Using RNAiso Blood, total RNA extraction can be completed in approximately 1 hour. Because the resulting total RNA will be almost completely free of DNA and protein, it can be used directly in downstream applications including RT-PCR, Northern blot analysis, mRNA isolation, and in vitro translation reactions

Application
expand_less
expand_more
Documents
expand_less
expand_more