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RNAiso Blood

RNAiso Blood

RNAiso Blood is a reagent for efficient and rapid RNA isolation from human or animal blood samples or those with high water content. The process involves adding RNAiso Blood solution to disrupt cells, followed by chloroform. After mixing and centrifugation, the solution separates into three layers: RNA in the upper transparent water layer, DNA in the middle layer, and proteins, polysaccharides, fatty acids, and cell debris in the red organic solvent layer at the bottom. The RNA is collected from the upper layer and recovered using isopropanol precipitation. The extraction process is completed in about 1 hour, and the RNA is suitable for applications like RT-PCR, Northern blotting, mRNA separation, and in vitro translation. The red organic solvent layer makes it easy to distinguish the layers. For RT-PCR, it’s recommended to treat RNA with Recombinant DNase I (RNase-free) to eliminate any gDNA contamination that could affect results.

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RNAiso Blood

  • • Effective total RNA extraction from samples containing a large amount of water, such as blood
  • • RNA extraction using AGPC (Acid Guanidinium-Phenol-Chloroform)

RNAiso Blood

RNAiso Blood (Code 9112)* 100 ml
RNAiso Blood (Code 9113)* 200 ml
  • * Since it contains protein denaturants, be careful not to let it come into contact with skin or clothing. If it gets into your eyes or on your skin, immediately wash with plenty of running water and seek medical advice.

RNAiso Blood

  • 4℃ (shade preservation)

RNAiso Blood

  • - Chloroform

    - Isopropanol

    - 75% ethanol [ prepared using DEPC-treated H2O] -

    RNase-free water

Type and Quantity of Sample RNAiso Blood Usage (ml)
0.01 *2 ~0.25 ml liquid sample (blood, biological fluids, fruit juice, etc.) 3 times the liquid sample volume. (If 0.25 ml sample was used, 0.75 ml RNAiso Blood was used)
  • *1 For plant samples containing a lot of polysaccharides, we recommend using the pretreatment reagent Fruit-mate™ for RNA Purification (Code 9192) .
  • *2 For extraction from trace samples, we recommend using the Dr. GenTLE™ Precipitation Carrier (Code 9094) together with isopropanol precipitation .

RNAiso Blood

Sample Sample Quantity Total RNA Extraction Amount
Human Whole Blood 0.25 ml 1~10 μg
Rat Whole Blood 0.25 ml 1~10 μg
Whole Cow Blood 0.25 ml 1~10 μg
Carp Whole Blood 0.25 ml 10~100 μg
Spinach Leaves 50 mg 30~60 μg
Tomato Fruit 50 mg 1~10 μg
Orange Fruit 50 mg 1~10 μg

RNAiso Blood