PrimeScript™ 1st strand cDNA Synthesis Kit

The PrimeScript 1st strand cDNA Synthesis Kit contains all the reagents necessary to synthesize first strand cDNA from total or polyA+ RNA using PrimeScript RTase, a MMLV (Moloney Murine Leukemia Virus)-derived reverse transcriptase. This enzyme is capable of synthesizing cDNA up to 12 kb in length. PrimeScript RTase can synthesize cDNA efficiently at 42℃, even from RNA templates that contain GC-rich regions or complex secondary structures, making it unnecessary to perform reactions at high temperatures that may degrade RNA. The first strand cDNA synthesized with this kit can be used for variety of applications, including second strand synthesis, hybridization, PCR amplification, and real-time PCR.

Overview

This cDNA synthesis kit includes oligo dT and random 6-mer primers
Strong strand displacement and extension capability: PrimeScript RT is capable of synthesizing long and full-length cDNA molecules (up to 12 kb)
High specificity: synthesize cDNA at 42°C—a temperature that does not promote RNA degradation—resulting in low background and higher yields
Outstanding accuracy: lowest error rate among five commercially available reverse transcriptases tested
Easy to use: low rates of non-specific annealing, even on incompletely denatured RNA
Works on challenging templates: excellent results even with templates that are GC-rich or have high levels of secondary structure
Highly sensitive: use less of your precious RNA samples
Demonstrated success with RT reaction times of 30 min and 60 min (for longer transcripts)

Resources

Application

1. First-strand cDNA synthesis
2. cDNA probe preparation
3. RT-PCR
4. Synthesis of cDNA libraries with a high proportion of full-length cDNAs

Citations

Materials and methods for the manufacture of pluripotent stem cells Iqbal S. Grewal, Rajkumar Ganesan, Sanjaya Singh us11981932 | 2024 May 14 | Read Patent "Total RNA was isolated from iPSC colonies and whole PBMCs using the RNeasy Plus mini kit (cat #74134, Qiagen) pursuant to the manufacturer's instructions.Total RNA was isolated from iPSC colonies and whole PBMCs using the RNeasy Plus mini kit (cat #74134, Qiagen) pursuant to the manufacturer's instructions.. Isolated RNA was quantified on nanodrop. cDNA synthesis was performed using the Primescript 1st strand cDNA synthesis kit (cat #6110B, Takara) pursuant to the manufacturer's instructions.. The resulting cDNA was used as a template for carrying out RT-PCR using primers listed in Table 6 above.The resulting cDNA was used as a template for carrying out RT-PCR using primers listed in Table 6 above." Less...