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PrimeDirect Probe RT-qPCR Mix with UNG

PrimeDirect Probe RT-qPCR Mix with UNG

PrimeDirect Probe RT-qPCR Mix, with UNG is designed for one-step real-time RT-PCR via probe detection (5'-nuclease method). This product is supplied as a 2X premix to facilitate easy preparation of reaction mixtures, requiring only the addition of your primer, probe, and sample. The RT-qPCR can be performed by simply adding your sample directly to the reaction mixture without intervening nucleic acid purification steps. The protocol for performing all steps in a single tube, from extraction of the nucleic acid to reverse transcription and qPCR, greatly reduces hands-on time and risk of contamination. The continuous reaction from nucleic acid extraction (90℃) through reverse transcription (55 to 65℃) is also suitable for the detection of RNA viruses with complex higher-order structures.

Pack Size

RR651A

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PrimeDirect Probe RT-qPCR Mix with UNG

Brand Catalogue Number Pack Size
PrimeDirect Probe RT-qPCR Mix with UNG RR651A 200 Tests
Components (200 reactions, 25 μl volume per reaction) Details
PrimeDirect Probe RT-qPCR Mix, with UNG (2X) 625 μl x 4
RNase Free H2O 1.25 ml x 2
ROX Reference Dye (50X conc.)* 100 μl
ROX Reference Dye II (50X conc.)* 100 μl

* When using a device that corrects the fluorescence signal between wells, such as Applied Biosystems’ real-time PCR devices, add at 0.5 times the final concentration.

 
  • Storage: -20°C
  • Features:
    • 2X for probe-based one-step RT-qPCR
    • Direct nucleic acid extraction from biological specimens in the reaction mix
    • High-temperature reverse transcription (55 - 65℃)
    • Highly resistant to various PCR inhibitors
    • Inclusion of UNG avoids false positives due to carryover contamination.

PrimeDirect Probe RT-qPCR Mix with UNG - Protocol and Downloads

Document Download Link
PrimeDirect Probe RT-qPCR Mix with UNG - Protocol Download User Manual
PrimeDirect Probe RT-qPCR Mix with UNG - Certificate of Analysis Download CoA - RR651A