Multiplex PCR allows simultaneous amplification of more than one PCR product by inclusion of multiple primer pairs in one PCR reaction. In addition to consuming fewer reagents, multiplex PCR facilitates time savings by simultaneous product amplification and makes it possible to use valuable samples efficiently.

The Multiplex PCR Assay Kit Ver.2 includes enzymes for high-speed priming and buffer composition optimized to increase the specificity of primer annealing. When compared to conventional multiplex PCR, this kit facilitates rapid, highly specific PCR amplification with minimal sequence-specific amplification bias. Depending on enzyme quantity and thermal cycling conditions, multiplex PCR with approximately 200 primer pairs may be performed. If desired, this kit can also be used for nonmultiplex PCR amplification using otherwise problematic primer pairs that frequently generate extra bands, smears, or fail to amplify the target fragment, because the Multiplex PCR Assay Kit Ver.2 promotes higher specificity while requiring less experimental time for optimization of PCR conditions.

SKU:RR062A