DTI UNG 25 U/µl - Glycerol Free
DTI UNG 25 U/µl - Glycerol Free
DTI Uracil DNA Glycosylase(UNG), Heat-labile, (Glycerol Free) (25 U/ μl) hydrolyzes N-glycosylic bonds between the deoxyribose sugars and the uracil bases in uracil-containing DNA leaving apyrimidinic sites in the DNA. UNG excises uracil from both single- and double-stranded dU-containing DNA but not from RNA. The high concentration glycerol free version makes the product suitable for lyophilisation.
SKU:
DTI UNG 25 U/µl - Glycerol Free
| Brand | Catalogue Number | Pack Size |
|---|---|---|
| DTI UNG 25 U/µl - Glycerol Free | DT0106.250 | 250 U |
Components
| Component | Details |
|---|---|
| DTI Uracil DNA Glycosylase (UNG), Heat-labile, (Glycerol Free) (25 U/μl) | 10 µl |
| Customized Pack Size | Available |
Storage Buffer
| Component | Concentration |
|---|---|
| Tris-HCl, pH 8.0 (at 4℃) | 20 mM |
| KCl | 100 mM |
| DTT | 1 mM |
| EDTA | 0.1 mM |
| NP-40 | 0.5% (v/v) |
| Tween 20 | 0.5% (v/v) |
- Storage and Shipment Conditions - Store at -80°C.
- Source Produced in E. coli strain expressing a recombinant Xiphophorus maculatus UNG mutant.
- Unit Definition One unit of UNG is defined as the amount of enzyme required to digest 1 μg of uracil-containing dsDNA at 25℃ in 30 min.
- Application Used in further manufacturing and lyophilization processes for PCR, RT-PCR, and LAMP assays.
- Inactivation by Heat The enzyme is completely and irreversibly inactivated by heat incubating at 50℃ for 10 min.
- Usage Protocol
• Use as per the requirement. Can be diluted if needed.
• Add suitable enzyme amounts based on application (typically 0.1 - 1 U/50μl reaction volume).
• UNG activation: Incubate for 10 min at 25℃.
• UNG inactivation: Incubate for 2 min at 95℃.
• Follow the specific protocol for application. - Quality Control Data Please refer to the certificate of analysis for each lot.
DTI UNG 25 U/µl - Glycerol Free - Protocol and Downloads
| Document | Download Link |
|---|---|
| DTI UNG 25 U/µl - Glycerol Free - Protocol | Download User Manual |
| DTI UNG 25 U/µl - Glycerol Free - Certificate of Analysis | Download CoA - DT0106.250 |
| DTI UNG 25 U/µl - Glycerol Free - Promotional Document | Download Flyer |
Experimental Sample - Performance Data
- Experimental Sample Performance data: qPCR reactions was performed with two qPCR reaction mix: One with UNG at a concentration of 2 U/ μl and other without UNG. Both these mixes were spiked with dU-containing amplicons, mimicking carryover contamination. The reaction mix without UNG generated a regular amplification curve, while the reaction mix containing UNG degraded the dU-containing amplicons resulting in significant reduction in amplification from the mimicking carryover contamination.