DTI High Conc. T4 DNA Ligase (2000 U/μl)
DTI High Conc. T4 DNA Ligase (2000 U/μl)
DTI High Conc. T4 DNA Ligase 2000 U/µl is derived from Phage T4 DNA. This enzyme catalyzes the formation of phosphodiester bonds between 3'-OH termini and 5'-P termini to join DNA strands. The molecular weight of T4 DNA Ligase is ~62,000 Da. For ligation, the optimal reaction pH is 7.6, and T4 DNA Ligase requires Mg2＋ and ATP as cofactors. This enzyme can ligate molecules with either cohesive ends or blunt ends.
SKU:DT1101.40K
DTI High Conc. T4 DNA Ligase (2000 U/μl)
| Brand | Catalogue Number | Pack Size |
|---|---|---|
| DTI High Conc. T4 DNA Ligase (2000 U/μl) | DT1101.40K | 2000 U |
Storage and Shipment Conditions
-20°C
Components
| Component | Pack Size |
|---|---|
| DTI High Conc. T4 DNA Ligase 2000 U/µl | 20 µl |
| 5X Ligation Reaction Buffer | 1 ml |
| * Customized pack size available |
Composition of Supplied Buffer (5X Ligation Reaction Buffer)
| Component | Concentration |
|---|---|
| Tris-HCl, pH 7.6 | 330 mM |
| MgCl2 | 50 mM |
| DTT | 5 mM |
| ATP | 5 mM |
| PEG#6000 | 37.5% |
- Source Escherichia coli carrying the plasmid that encodes the gene of T4 DNA ligase.
- Unit Definition One unit is the amount of enzyme that ligates more than 90% of 6 μg of λDNA-Hind III fragments in a 20 μl mixture in 30 minutes at 16°C. One unit of this enzyme corresponds to 0.008 Weiss units by the ATP-PPi exchange reaction in 66 mM Tris-HCl, pH 7.6 that contains 6.6 mM MgCl2, 10 mM DTT, 66 μM ATP, and 3.3 μM [32P]-Na4P2O7.
- Applications
- Insertion of a DNA fragment into a vector
- Linker or adaptor ligation to a DNA fragment
DTI High Conc. T4 DNA Ligase 2000 U/µl - Protocol and Downloads
| Document | Download Link |
|---|---|
| DTI High Conc. T4 DNA Ligase 2000 U/µl - Protocol | Download User Manual |
| DTI High Conc. T4 DNA Ligase 2000 U/µl - Certificate of Analysis | Download CoA - DT1101.40K |
| DTI High Conc. T4 DNA Ligase 2000 U/µl - Promotional Document | Download Flyer |
Experimental Sample
Efficient ligation of λ HIND III DNA obtained using the enzyme and buffer.
Band of 2027 bp was clearly identified by agarose gel electrophoresis. Below is the image of the same.
Figure 1: Efficient ligation of λ HIND III DNA with a 2027 bp band clearly identified by agarose gel electrophoresis.