Skip to product information
1 of 1

DTI High Conc. T4 DNA Ligase (2000 U/μl)

DTI High Conc. T4 DNA Ligase (2000 U/μl)

DTI High Conc. T4 DNA Ligase 2000 U/µl is derived from Phage T4 DNA. This enzyme catalyzes the formation of phosphodiester bonds between 3'-OH termini and 5'-P termini to join DNA strands. The molecular weight of T4 DNA Ligase is ~62,000 Da. For ligation, the optimal reaction pH is 7.6, and T4 DNA Ligase requires Mg2+ and ATP as cofactors. This enzyme can ligate molecules with either cohesive ends or blunt ends.

Pack Size

SKU:DT1101.40K

View full details

DTI High Conc. T4 DNA Ligase (2000 U/μl)

Brand Catalogue Number Pack Size
DTI High Conc. T4 DNA Ligase (2000 U/μl) DT1101.40K 2000 U

Storage and Shipment Conditions

-20°C

Components

Component Pack Size
DTI High Conc. T4 DNA Ligase 2000 U/µl 20 µl
5X Ligation Reaction Buffer 1 ml
* Customized pack size available  

Composition of Supplied Buffer (5X Ligation Reaction Buffer)

Component Concentration
Tris-HCl, pH 7.6 330 mM
MgCl2 50 mM
DTT 5 mM
ATP 5 mM
PEG#6000 37.5%
  • Source Escherichia coli carrying the plasmid that encodes the gene of T4 DNA ligase.
  • Unit Definition One unit is the amount of enzyme that ligates more than 90% of 6 μg of λDNA-Hind III fragments in a 20 μl mixture in 30 minutes at 16°C. One unit of this enzyme corresponds to 0.008 Weiss units by the ATP-PPi exchange reaction in 66 mM Tris-HCl, pH 7.6 that contains 6.6 mM MgCl2, 10 mM DTT, 66 μM ATP, and 3.3 μM [32P]-Na4P2O7.
  • Applications
    • Insertion of a DNA fragment into a vector
    • Linker or adaptor ligation to a DNA fragment

DTI High Conc. T4 DNA Ligase 2000 U/µl - Protocol and Downloads

Document Download Link
DTI High Conc. T4 DNA Ligase 2000 U/µl - Protocol Download User Manual
DTI High Conc. T4 DNA Ligase 2000 U/µl - Certificate of Analysis Download CoA - DT1101.40K
DTI High Conc. T4 DNA Ligase 2000 U/µl - Promotional Document Download Flyer

Experimental Sample

Efficient ligation of λ HIND III DNA obtained using the enzyme and buffer.

Band of 2027 bp was clearly identified by agarose gel electrophoresis. Below is the image of the same.

Efficient ligation of λ HIND III DNA

Figure 1: Efficient ligation of λ HIND III DNA with a 2027 bp band clearly identified by agarose gel electrophoresis.