DTI FabTaq HS-Glycerol Free (50U/ul)
DTI FabTaq HS-Glycerol Free (50U/ul)
DTI FabTaq HS-Glycerol Free (50 U/ul) is a hot start PCR enzyme derived from Thermus aquaticus that includes a neutralizing monoclonal antibody that recognizes Taq DNA polymerase. This antibody binds to Taq DNA polymerase and prevents non-specific amplification due to mispriming and/or formation of primer dimers before thermal cycling. The antibody is denatured during the initial DNA-denaturation step, allowing this product to be used with standard PCR conditions. Since the product is glycerol free, it is suitable for lyophilization processes
SKU:DT0103.2500
DTI FabTaq HS-Glycerol Free (50 U/μl)
| Brand | Catalogue Number | Pack Size |
|---|---|---|
| DTI FabTaq HS-Glycerol Free (50 U/μl) | DT0103.2500 | 2500 U |
- Storage and Shipment Conditions -80°C
- Components
Components Quantity DTI FabTaq HS-Glycerol Free (50 U/μl) 50 µl * Customized pack size available - Materials required but not provided:
- 10X PCR buffer
- 25 mM/50 mM MgCl2
- 2.5 mM dNTP
- Template DNA/cDNA
- Target specific forward and reverse primers
- Nuclease free water
- Thermal cycler
- Agarose electrophoresis system
- Loading dyes and marker
- Unit Definition One unit is the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble products in 30 minutes at 74°C with activated salmon sperm DNA as the template-primer.
- Application
- For DNA amplification by hot start PCR
- For DNA sequencing
- For further manufacturing and lyophilization processes
- Reaction mixture for unit definition :
25 mM TAPS (pH 9.3 at 25℃) 50 mM KCl 2 mM MgCl2 0.1 mM DTT 200 μM each dATP·dGTP·dCTP 100 μM [3H]-dTTP 0.25 mg/ml activated salmon sperm DNA - Purity Nicking, endonuclease, and exonuclease activity were not detected after incubation of 0.6 μg of supercoiled pBR322 DNA, 0.6 μg of λDNA, or 0.6 μg of λ-Hin d III digest with 10 U of this enzyme for 1 hour at 74°C.
- Quality Control Data Please see the certificate of analysis for each lot.
DTI FabTaq HS-Glycerol Free (50U/ul) - Protocol and Downloads
| Document | Download Link |
|---|---|
| DTI FabTaq HS-Glycerol Free (50U/ul) - Protocol | Download User Manual |
| DTI FabTaq HS-Glycerol Free (50U/ul) - Certificate of Analysis | Download CoA - DT0103.2500 |
| DTI FabTaq HS-Glycerol Free (50U/ul) - Promotional Document | Download Flyer |
Results
Experimental Sample
a) Amplification data: Good performance of this product has been confirmed by PCR using λ DNA as template (amplified fragment size 8 kbp). The protocol used for the assay and the results are as follows:
Figure 1: Amplification data showing product performance with λ DNA as template.
Figure 2: Amplification data for λ DNA (8 kbp amplified fragment size).
b) Hot start function data: Test target sample added to a PCR system containing FabTaq and FabTaq HS respectively. Following an incubation at RT for 30 mins, the products were amplified using a thermal cycler. Non-specific bands (around 280 bp) were observed in the PCR system along with specific target bands (838 bp) using FabTaq, while there were no non-specific bands observed in PCR system using FabTaq HS.
Figure 3: Additional data showcasing product performance.
Figure 4: Hot start function data showing non-specific bands in PCR system with FabTaq and absence of non-specific bands with FabTaq HS.