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    DSS Takara Bio

    DTI Agarose Routine

    DTI Agarose Routine

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    Pack Size

    DT1701.500

    Agarose electrophoresis is the most effective way to identify and separate DNA fragments. The purity of agarose directly affects the resolution of DNA and the clarity of electrophoresis results. If the agarose contains sugars, salts, proteins, it will affect the migration speed of DNA in the gel and subsequently the reaction performance of recovered DNA fragments during enzymatic reactions. Hence, using high quality agarose is important for success of the experiment.

    DTI Agarose routine is a high-quality agarose product, suitable for making 1-3% agarose gel. When stained with EtBr, the electrophoretic separation performance is strong, bands are clear and is suitable for electrophoresis of various DNA fragments. It is an economical and ideal grade for conventional agarose electrophoresis.

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    DTI Agarose Routine

    Brand Catalogue Number Pack Size
    DTI Agarose Routine DT1701.500 500 g
    • Gel Strength (1.5% gel) >800 g/cm²
    • Gelling temperature 35°-40° C
    • Electroendosmosis 0.09-0.12
    • Sulfate content <0.2%

    DTI Agarose Routine - Protocol and Downloads

    Document Download Link
    DTI Agarose Routine - Protocol Download User Manual
    DTI Agarose Routine - Certificate of Analysis Download CoA - DT1701.500
    DTI Agarose Routine - Promotional Document Download Flyer

    Experimental Sample - DNA Marker Run

    Experimental sample: The below image indicates the run of different DNA markers as indicated in the table, on a 2% TAE gel.

    DNA Marker Run on 2% TAE Gel
    Lane # Description Fragment Size for Reference
    1,2 Cat#DT1801.100, DTI 100bp Ladder (Dye Plus) 1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100 base pairs
    3,4 Cat#DT1803.100, DTI2000 DNA Ladder (Dye Plus) 2000, 1000, 750, 500, 250, 100 base pairs
    5,6 Cat#3428A, DL5000 DNA Ladder, Fragment Size (bp) 5000, 3000, 2000, 1500, 1000, 750, 500, 250, 100 base pairs
    7,8 Cat#DT1802.100, DTI 1 kb Ladder (Dye Plus) 10, 6, 5, 4, 3, 2, 1 Kilo base pairs