1 0

    DTI Agarose Premium

    DTI Agarose Premium

    Agarose electrophoresis is the most effective way to identify and separate DNA fragments. The purity of agarose directly affects the resolution of DNA and the clarity of electrophoresis results. If the agarose contains sugars, salts, or proteins, it will affect the migration speed of DNA in the gel and subsequently the reaction performance of recovered DNA fragments during enzymatic reactions. Hence, using high-quality agarose is important for the success of the experiment. DTI Agarose premium is a high-quality agarose product with high gel strength, suitable for making as low as 0.7% to as high concentration 3% agarose gel. When stained with EtBr, the electrophoretic separation performance is strong, bands are clear with minimal background, and it is suitable for electrophoresis of various DNA fragments. It is an economical and ideal grade for conventional agarose electrophoresis.

    Pack Size

    DT1702.500

    View full details

    DTI Agarose Premium

    Brand Catalogue Number Pack Size
    DTI Agarose Premium DT1702.500 500 g
    • Gel Strength (1.0% gel) >1200 g/cm²
    • Gelling temperature 34.5°-37.5° C
    • Electroendosmosis 0.05-0.13
    • Sulfate content <0.1%

    DTI Agarose Premium - Protocol and Downloads

    Document Download Link
    DTI Agarose Premium - Protocol Download User Manual
    DTI Agarose Premium - Certificate of Analysis Download CoA - DT1702.500

    Experimental Sample - DNA Marker Run

    Comparative runs of DNA markers on DT1702.500 Agarose premium vs DT1701.500 Agarose Routine.

    a)  λ Hind III digest DNA Marker run on 0.7% Agarose gel made using TAE buffer

    DNA Marker Run on 2% TAE Gel
    Lane # Description Fragment Size for Reference
    1,2 Cat# 3403, λ Hind III digest DNA Marker 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125. The cohesive ends (12b cos site of bacteriophage lambda) of fragments 23130 bp and 4361 bp may be separated by heating to 65 °C for 5 min.

    b)  100 bp DNA Marker run on 3% Agarose gel made using TAE buffer

     

    DNA Marker Run on 2% TAE Gel
    Lane # Description Fragment Size for Reference
    1,2 Cat#DT1801.100, DTI 100bp Ladder (Dye Plus) 1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100 base pairs