DNA Ligation Kit: Mighty Mix

The DNA Ligation Kit, Mighty Mix is a 2X ligation premix for high-efficiency ligations, especially for blunt-ended ligations. This DNA ligation kit can also be used for ligation into TA-vectors. The 2X ligation mix is compatible with small ligation reaction volumes (10 µl) for when DNA quantity is a limiting factor, and it contains sufficient reagent for 75–150 reactions. The reaction mixtures from this DNA ligation kit can be used directly in bacterial transformation

Application

-Highly efficient and fast DNA ligation
-Small reaction volume ligation -TA and blunt-end cloning

Citations

Hayashi, K., Nakazawa, M., Ishizaki, Y., Hiraoka, N. & Obayashi, A. Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol. Nucleic Acids Res. 14, 7617–31 (1986).

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