QuickCut™ Afa I (Rsa I)

QuickCut restriction enzyme is a kind of enzyme which can quickly digest substrate DNA. The digestion activity of each QuickCut enzyme can reach 100% in the 10X QuickCut Buffer or 10X QuickCut Green Buffer, and the substrate DNA, such as plasmid DNA and PCR product, can be cut completely in 5 to 30 min. In addition, 10X QuickCut Buffer and 10X QuickCut Green Buffer are available for other enzymes also in QuickCut
series. Then some kinds of the QuickCut enzymes can be reacted simultaneously in one reaction. So QuickCut enzyme can lead easy operation, time saving, and elimination of the complex operation in restriction enzyme digestion. Each QuickCut enzyme is supplied together with two kinds of universal buffer: 10X QuickCut Buffer and 10X QuickCut Green Buffer. 10X QuickCut Green Buffer contains composition of the sample loading buffer for agarose gel electrophoresis in 10X QuickCut Buffer, so the digested DNA can be used for electrophoresis directly. This buffer contains two tracking dyes and the migration speed of the blue dye is equivalent to that of 3-5 kb DNA fragment and the yellow dye is to 10 bp DNA fragment in 1% agarose gel. 

Resources:

 -Download Manual