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DTI Uracil DNA Glycosylase(UNG), Heat-labile, (Glycerol Free) (2 U/ul) hydrolyzes N-glycosylic bonds between the deoxyribose sugars and the uracil bases in uracil-containing DNA leaving apyrimidinic sites in the DNA. UNG excises uracil from both single- and double-stranded dU-containing DNA but not from RNA. The glycerol free version makes the product suitable for lyophilisation.

Specifications

Documents

Applications

Brand	Catalogue Number	Pack Size
DTI UNG 2 U/μl - Glycerol Free	DT0105.20	20 U

➥ Storage and Shipment Conditions:

-80°C

➥ Components:

Components	Quantity
DTI Uracil DNA Glycosylase (UNG), Heat-labile, (Glycerol Free) (2U/ul)	10 µl

* Customized pack size available

➥ 5) Storage Buffer:

20 mM	Tris-HCl, pH 8.0 (at 4℃)
100 mM	KCI
1 mM	DTT
0.1 mM	EDTA
0.5% (v/v)	NP-40
0.5% (v/v)	Tween 20

➥ Source:

Produced in E. coli strain expressing a recombinant Xiphophorus maculatus UNG mutant.

➥ Unit definition:

One unit of UNG is defined as the amount of enzyme required to digest 1 μg of uracil-containing dsDNA at 25℃ in 30 min.

➥ Application:

• Further manufacturing and lyophilization processes for PCR, RT-PCR and LAMP assays

➥ Inactivation by heat:

The enzyme is completely and irreversibly inactivated by heat incubating at 50℃ for 10 min.

➥ Usage protocol:

• Add suitable amount of enzyme based on the application. Generally, a range of 0.1 - 1 U/50μl of reaction volume is

used

• UNG activation: Incubate for 10 min at 25℃

• UNG inactivation: Incubate for 2 min at 95℃

• Follow with the protocol of application

➥ Quality control data: Please see the certificate of analysis for each lot

DTI UNG 2 U/μl - Glycerol Free - Protocol

DTI UNG 2 U/μl - Glycerol Free - Certificate of Analysis

DTI UNG 2 U/μl - Glycerol Free - Promotional Document

Experimental Sample

Performance data: qPCR reactions was performed with two qPCR reaction mix: One with UNG at a concentration of 2 U/ μl and other without UNG. Both these mixes were spiked with dU-containing amplicons, mimicking carryover contamination. The reaction mix without UNG generated a regular amplification curve, while the reaction mix containing UNG degraded the dU-containing amplicons resulting significant reduction in amplification from the mimicking carryover contamination.

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