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DTI JadeAmp Max HiFid Taq Premix
The DTI JadeAmp Max HiFid Taq Premix PCR Master Mix offers a powerful and convenient option for high-yield, routine, and specific PCR. This premix formulation includes an optimized buffer, PCR enzyme, dNTP mixture, gel-loading dye (green), and a density reagent in a convenient 2X premix format. The buffer is optimized for better performance with AT or GC-rich targets, and allows amplification of long products. It is possible to amplify 15 kb genomic DNA fragments with this master mix.
Only primers and DNA template need to be added to initiate the reaction. Completed reactions can be analyzed directly via gel electrophoresis. The vivid green dye separates into blue and yellow dye fronts when the PCR product is run on an agarose gel.
Brand | Catalogue Number | Pack Size |
DTI JadeAmp Max HiFid Taq Premix | DT0202.80 | 80 rxn (25 μl volume) |
DTI JadeAmp Max HiFid Taq Premix | DT0202.320 | 320 rxn (25 μl volume) |
• Ease-of-use—emerald green loading dye tracks migration during electrophoresis.
• High yield—obtain at least 10 times more end product.
• Long PCR—amplify targets up to 10 kb in length.
• Easy integration with downstream application—perform restriction digests directly in the PCR buffer or use in TA cloning.
DTI JadeAmp premixes outperforms Competitor(C) Red mix for GC-rich targets
Results
Amplification of GC-rich targets
Comparison of hot-start premixes
Conclusions
DTI JadeAmp FabTaq Premix and DTI JadeAmp Max HiFid Taq Premix (non-hot-start and hot-start) outperformed Competitor(C) Red Mix in background amplification, target GC-content, and target length. The DTI JadeAmp series gave minimal background amplification and better performance on GC-rich targets. Additionally, the DTI JadeAmp Max HiFid Taq Premix master mixes were able to amplify long targets while Competitor(C) Red Mix failed to amplify the 4-kb target gene TGFB-1.
With DTI's JadeAmp series of PCR master mixes, you can streamline your genotyping workflow without worrying about GC content of your target or nonspecific background amplification.
DTI JadeAmp FabTaq Premix can amplify targets up to 10 kb in length, including targets that are GC- or AT-rich. Additionally, PCR products generated with DTI JadeAmp FabTaq Premix can be used directly for restriction enzyme digestion, sequencing, or TA-cloning without need for further purification.
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