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The DTI JadeAmp Max HiFid Taq Premix PCR Master Mix offers a powerful and convenient option for high-yield, routine, and specific PCR. This premix formulation includes an optimized buffer, PCR enzyme, dNTP mixture, gel-loading dye (green), and a density reagent in a convenient 2X premix format. The buffer is optimized for better performance with AT or GC-rich targets, and allows amplification of long products. It is possible to amplify 15 kb genomic DNA fragments with this master mix.

Only primers and DNA template need to be added to initiate the reaction. Completed reactions can be analyzed directly via gel electrophoresis. The vivid green dye separates into blue and yellow dye fronts when the PCR product is run on an agarose gel.

Specifications

Documents

Applications

FAQs

Brand	Catalogue Number	Pack Size
DTI JadeAmp Max HiFid Taq Premix	DT0202.80	80 rxn (25 μl volume)
DTI JadeAmp Max HiFid Taq Premix	DT0202.320	320 rxn (25 μl volume)

• Streamlined workflow—simply add template, primers, and water to the 2X master mix then load reactions directly onto gel.

• Ease-of-use—emerald green loading dye tracks migration during electrophoresis.

• High yield—obtain at least 10 times more end product.

• Long PCR—amplify targets up to 10 kb in length.

• Easy integration with downstream application—perform restriction digests directly in the PCR buffer or use in TA cloning.

DTI JadeAmp Max HiFid Taq Premix - Protocol

DTI JadeAmp Max HiFid Taq Premix - Certificate of Analysis

DTI JadeAmp Max HiFid Taq Premix - Promotional Document

DTI JadeAmp premixes outperforms Competitor(C) Red mix for GC-rich targets

Results

Amplification of GC-rich targets

Our results showed that the non-hot-start DTI JadeAmp FabTaq Premix and DTI JadeAmp Max HiFid Taq Premix gave minimal background amplification with GC-rich targets. Non-hot-start Competitor(C) Red Mix, on the other hand, resulted in nonspecific amplification and was unable to amplify the target with a high GC content (72.3%). While DTI JadeAmp FabTaq Premix failed to amplify the TGFB-1 gene that is 4 kb long with a 63.1% GC content, DTI JadeAmp Max HiFid Taq Premix was able to generate a clean and distinct band. We were unable to amplify the 4-kb TGFB-1 target using Competitor(C) Red Mix.

Figure 1: Comparison of amplification profiles of four GC-rich target genes using DTI JadeAmp FabTaq Premix, DTI JadeAmp Max HiFid Taq Premix, or Competitor(C) Red Mix. 10 µl from each PCR reaction was loaded on a 1% agarose gel.

Comparison of hot-start premixes

The performance of hot-start, dye-added premixes, DTI JadeAmp Max HiFid Taq Premix and Competitor(C) Red Mix, was compared using GC-rich targets. DTI JadeAmp Max HiFid Taq Premix was able to amplify all expected products with minimal nonspecific background amplification—including long targets, regardless of GC content. In contrast, Competitor(C) Red Mix generated background amplification and failed to amplify the high GC-content gene TGFB-1, as seen in the figure below.

Figure 2: Amplification of four GC-rich targets using hot-start premixes DTI JadeAmp Max HiFid Taq Premix and Competitor(C) Red Mix. 10 µl of PCR products from each reaction were loaded on a 1% agarose gel.

Conclusions

DTI JadeAmp FabTaq Premix and DTI JadeAmp Max HiFid Taq Premix (non-hot-start and hot-start) outperformed Competitor(C) Red Mix in background amplification, target GC-content, and target length. The DTI JadeAmp series gave minimal background amplification and better performance on GC-rich targets. Additionally, the DTI JadeAmp Max HiFid Taq Premix master mixes were able to amplify long targets while Competitor(C) Red Mix failed to amplify the 4-kb target gene TGFB-1.

With DTI's JadeAmp series of PCR master mixes, you can streamline your genotyping workflow without worrying about GC content of your target or nonspecific background amplification.

Which enzymes faciliate efficient workflow when analyzing multiple samples by gel electrophoresis after PCR (e.g., genotyping screens)?

We recommend DTI JadeAmp FabTaq Premix (Cat.# DT0201.320). This product is completely premixed, making it easy to prepare PCR reaction mixtures which can be loaded directly on an agarose gel for electrophoresis after the reaction. For an efficient workflow with minimal pipetting, DTI JadeAmp FabTaq Premix contains a green tracking dye. Additionally, density agent is included to aid in gel loading.

DTI JadeAmp FabTaq Premix can amplify targets up to 10 kb in length, including targets that are GC- or AT-rich. Additionally, PCR products generated with DTI JadeAmp FabTaq Premix can be used directly for restriction enzyme digestion, sequencing, or TA-cloning without need for further purification.

Which enzymes are suitable for colony PCR?

We recommend DTI CobaltAmp HiFid Taq HS Premix for colony PCR. These enzyme preparations tolerate the presence of substantial bacterial nucleic acid carry-over. Because tracking dye and density agent are included in these master mixes, the PCR reaction mixtures may be loaded directly on an agarose gel for electrophoresis.

Which enzymes are suitable for fast PCR?

DTI CobaltAmp HiFid Taq HS Premix is an economical choice for high-throughput projects. This enzyme is formulated to include high speed polymerase, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent in a 2X premix. Since it requires an extension time of only 10 sec/kb, colony PCR reactions can be completed in less than 1 hour for inserts up to 1 kb.

What precautions should be taken when using an inosine-containing primer?

DTI FabTaq and DTI FabTaq Hot Start Version enzymes are compatible with inosine-containing primers. It is important to note that inosine-containing primers should not be used with PCR enzymes that have 3’→5’ exonuclease activity. When using one of these PCR enzymes, we recommend using mixtures of degenerate primers with A, T, G, and C at the desired position(s) rather than inosine-containing primers when performing degenerate PCR.

What is the terminal structure of DTI FabTaq and DTI FabTaq Hot Start Version enzymes’ amplification product?

DTI FabTaq and DTI FabTaq Hot Start Version enzymes primarily yield amplification products containing 3’-dA overhangs, which facilitates TA cloning.

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