Home > Products > Modifying Enzymes > DTI High Conc. T4 DNA Ligase (2000 U/μl)

DTI High Conc. T4 DNA Ligase 2000 U/µl is derived from Phage T4 DNA. This enzyme catalyzes the formation of phosphodiester bonds between 3'-OH termini and 5'-P termini to join DNA strands. The molecular weight of T4 DNA Ligase is ~62,000 Da. For ligation, the optimal reaction pH is 7.6, and T4 DNA Ligase requires Mg2＋ and ATP as cofactors. This enzyme can ligate molecules with either cohesive ends or blunt ends.

Specifications

Documents

Applications

Brand	Catalogue Number	Pack Size
DTI High Conc. T4 DNA Ligase (2000 U/μl)	DT1101.40K	2000 U

➥ Storage and Shipment Conditions:

-20°C

➥ Components:

Components	Pack Size
DTI High Conc. T4 DNA Ligase 2000 U/µl	20 µl
5X Ligation reaction Buffer	1 ml

* Customized pack size available

➥ 5) Composition of Supplied Buffer

5X Ligation reaction Buffer

330 mM	Tris-HCl, pH 7.6
50 mM	MgCl2
5 mM	DTT
5 mM	ATP
37.5%	PEG#6000

➥ Source:

Escherichia coli carrying the plasmid that encodes the gene of T4 DNA ligase.

➥ Unit definition:

One unit is the amount of enzyme that ligates more than 90％ of 6 μg of λDNA-Hind III fragments in a 20 μl mixture in 30 minutes at 16℃. One unit of this enzyme corresponds to 0.008 Weiss units by the ATP-PPi exchange reaction1) in 66 mM Tris-HCl, pH7.6 that contains 6.6 mM MgCl2, 10 mM DTT, 66 μM ATP, and 3.3 μM [32P]-Na4P2O7.

➥ Application:

1. Insertion of a DNA fragment into a vector

2. Linker or adaptor ligation to a DNA fragment

DTI High Conc. T4 DNA Ligase 2000 U/µl - Protocol

DTI High Conc. T4 DNA Ligase 2000 U/µl - Certificate of Analysis

DTI High Conc. T4 DNA Ligase 2000 U/µl - Promotional Document

Experimental Sample

Efficient ligation of λ HIND III DNA obtained using the enzyme and buffer. Band of 2027 bp was clearly identified by agarose gel electrophoresis. Below is the image of the same.

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