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DTI FabTaq HS-Glycerol Free (50 U/ul) is a hot start PCR enzyme derived from Thermus aquaticus that includes a neutralizing monoclonal antibody that recognizes Taq DNA polymerase. This antibody binds to Taq DNA polymerase and prevents non-specific amplification due to mispriming and/or formation of primer dimers before thermal cycling. The antibody is denatured during the initial DNA-denaturation step, allowing this product to be used with standard PCR conditions. Since the product is glycerol free, it is suitable for lyophilization processes

Specifications

Documents

Applications

Brand	Catalogue Number	Pack Size
DTI FabTaq HS-Glycerol Free (50 U/ul)	DT0103.2500	2500 U

➥ Storage and Shipment Conditions:

-80°C

➥ Components:

Components	Quantity
DTI FabTaq HS-Glycerol Free (50 U/ul)	50 µl

* Customized pack size available

➥ Materials required but not provided:

• 10X PCR buffer

• 25 mM/50 mM MgCl2

• 2.5 mM dNTP

• Template DNA/cDNA

• Target specific forward and reverse primers

• Nuclease free water

• Thermal cycler

• Agarose electrophoresis system

• Loading dyes and marker

➥ Unit definition:

One unit is the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble products in 30 minutes at 74°C with activated salmon sperm DNA as the template-primer.

➥ Application:

• For DNA amplification by hot start PCR

• For DNA sequencing

• For further manufacturing and lyophilization processes

➥ Reaction mixture for unit definition :

25 mM	TAPS (pH 9.3 at 25℃)
50 mM	KCI
2 mM	MgCl2
0.1 mM	DTT
200 μM	each dATP･dGTP･dCTP
100 μM	[3H]-dTTP
0.25 mg/ml	activated salmon sperm DNA

➥ Purity:

Nicking, endonuclease, and exonuclease activity were not detected after incubation of 0.6 μg of supercoiled pBR322 DNA, 0.6 μg of λDNA, or 0.6 μg of λ-Hin d III digest with 10 U of this enzyme for 1 hour at 74°C.

➥ Quality control data: Please see the certificate of analysis for each lot

DTI FabTaq HS-Glycerol Free (50U/ul) - Protocol

DTI FabTaq HS-Glycerol Free (50U/ul) - Certificate of Analysis

DTI FabTaq HS-Glycerol Free (50U/ul) - Promotional Document

Experimental Sample

a) Amplification data: Good performance of this product has been confirmed by PCR using λ DNA as template (amplified fragment size 8 kbp). The protocol used for the assay and the results are as follows:

b) Hot start function data:

Test target sample added to a PCR system containing FabTaq and FabTaq HS respectively. Following an incubation at RT for 30 mins, the products were amplified using a thermal cycler. Non specific bands (around 280 bp) were observed in the PCR system along with specific target bands (838 bp) using FabTaq, while there were no non specific bands observed in PCR system using FabTaq HS.

The protocol used for the assay and the results are as follows:

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