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MightyAmp™ Genotyping Kit

MightyAmp™ Genotyping Kit

The MightyAmp Genotyping Kit offers a simple method of DNA extraction and PCR amplification for animal tissues (e.g., mouse tail), and plant tissues.
The Extraction Buffer in combination with Proteinase K included in the kit allow direct DNA extraction from plant and animal tissues. MightyAmp DNA Polymerase enables analysis of a wide range of target DNA (regardless of GC composition) from crude extracts containing PCR-inhibiting compounds that cause difficulty when using conventional PCR polymerases. MightyAmp DNA Polymerase is well suited for amplification of DNA up to approximately 2 kb in length, and shows good yield even with small template quantities. The 5X Loading Dye (included) is formulated to reduce the effect of crude-sample-derived contaminants, and facilitates rapid, easy analysis of PCR products by agarose gel electrophoresis.
MightyAmp DNA Polymerase is designed for hot-start PCR. It includes monoclonal antibodies to inhibit polymerase activity at temperatures up to 98℃, thereby preventing nonspecific amplification.

Pack Size

SKU:R074A

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MightyAmp™ Genotyping Kit

Brand Catalogue Number Pack Size
MightyAmp™ Genotyping Kit R074A 200 Rxns
Components (50-μl reactions, for 200 reactions) Details
MightyAmp DNA Polymerase (1.25 U/μl) 200 μl
2X MightyAmp Buffer (Mg2+, dNTP plus)* 1.25 ml x 4
5X Loading Dye 1 ml x 3
Extraction Buffer 20 ml
Proteinase K 200 μl

* Mg2+ concentration is 4 mM in 2X buffer. Each dNTP is provided at 800 μM in 2X buffer.

  • Storage: All other components : -20 ℃ 
    Extraction Buffer : Room temperature (15 - 25℃)

MightyAmp™ Genotyping Kit - Protocol and Downloads

Document Download Link
MightyAmp™ Genotyping Kit - Protocol Download User Manual
MightyAmp™ Genotyping Kit - Certificate of Analysis Download CoA - R074A