MightyAmp™ Genotyping Kit
MightyAmp™ Genotyping Kit
The MightyAmp Genotyping Kit offers a simple method of DNA extraction and PCR amplification for animal tissues (e.g., mouse tail), and plant tissues.
The Extraction Buffer in combination with Proteinase K included in the kit allow direct DNA extraction from plant and animal tissues. MightyAmp DNA Polymerase enables analysis of a wide range of target DNA (regardless of GC composition) from crude extracts containing PCR-inhibiting compounds that cause difficulty when using conventional PCR polymerases. MightyAmp DNA Polymerase is well suited for amplification of DNA up to approximately 2 kb in length, and shows good yield even with small template quantities. The 5X Loading Dye (included) is formulated to reduce the effect of crude-sample-derived contaminants, and facilitates rapid, easy analysis of PCR products by agarose gel electrophoresis.
MightyAmp DNA Polymerase is designed for hot-start PCR. It includes monoclonal antibodies to inhibit polymerase activity at temperatures up to 98℃, thereby preventing nonspecific amplification.
SKU:R074A

MightyAmp™ Genotyping Kit
| Brand | Catalogue Number | Pack Size |
|---|---|---|
| MightyAmp™ Genotyping Kit | R074A | 200 Rxns |
| Components (50-μl reactions, for 200 reactions) | Details |
|---|---|
| MightyAmp DNA Polymerase (1.25 U/μl) | 200 μl |
| 2X MightyAmp Buffer (Mg2+, dNTP plus)* | 1.25 ml x 4 |
| 5X Loading Dye | 1 ml x 3 |
| Extraction Buffer | 20 ml |
| Proteinase K | 200 μl |
* Mg2+ concentration is 4 mM in 2X buffer. Each dNTP is provided at 800 μM in 2X buffer.
- Storage: All other components : -20 ℃
Extraction Buffer : Room temperature (15 - 25℃)
MightyAmp™ Genotyping Kit - Protocol and Downloads
| Document | Download Link |
|---|---|
| MightyAmp™ Genotyping Kit - Protocol | Download User Manual |
| MightyAmp™ Genotyping Kit - Certificate of Analysis | Download CoA - R074A |