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E. coli HST08 Premium Competent Cells are an E. coli HST08 strain that lacks the gene group (mcrA, mrr – hsdRMS - mcrBC) responsible for cleaving foreign methylated DNAs. The cells are also an F– strain, which allows the use of BAC and fosmid vectors. In a transformation using a pUC plasmid, Stellar cells allow blue-white screening of recombinants with X-gal, based on β-galactosidase α-complementation. These cells display an excellent transformation potential even for large DNA fragments, making it easy to produce clones or libraries of fragments up to 20 kb.
