Real-time RT-PCR is made easy with the DTI FabScript One Step RT-PCR Reagents kit (Perfect Real Time), a one-step real-time RT-PCR kit designed for real-time RT-PCR (qPCR) via probe-based qPCR assays such as 5' nuclease assays. In this 5' nuclease assay realtime PCR (qPCR) kit, one-step real-time RT-PCR can be performed in a single tube, simplifying amplification and minimizing the risk of contamination. Amplified products are monitored in real time, so there is no need to verify them through electrophoresis following PCR. The sensitivity provided by the enzymes in this kit enables detection of very small amounts of RNA, including RNA derived from viral, rare, or precious samples.

Features:

• Enables accurate and rapid analysis of viral RNA or RNA derived from rare or precious sources.
• Contains HiFid Taq HS, a high-efficiency, hot-start PCR enzyme for PCR.
• Buffer system is real-time PCR optimized, increasing amplification efficiency and detection sensitivity.

Application:

• One-step real-time RT-PCR
• Probe-based qPCR assays such as 5' nuclease assays and real-time PCR (qPCR) assays

Resources:

• Download brochure
• Download User Manual