Takara Taq (r Taq DNA Polymerase without dNTPs)

Takara Taq DNA Polymerase is a recombinant version of full-length Taq polymerase. It has the same characteristics and capabilities as the native Taq polymerase, and is suitable for a variety of standard PCR applications. The polymerase is supplied with separate tube of buffer (Mg2+ plus).


Detail
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Product
Catalogue No.
Size

Takara Taq DNA Polymerase

R500A

250 Units

Storage
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-20℃

Applications
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1. For DNA amplification by PCR-well mini-gel, respectively.

2. For DNA sequencing

storage buffer
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Tris-HCl (pH8.0)

20 mM

KCI

100 mM

EDTA

0.1 mM

0.2 mM DTT

1 mM

Tween 20

0.5% 

Nonidet P-40

0.5%

Glycerol

50%

Unit definition
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One unit is the amount of enzyme that will incorporate10 nmol of dNTP into acid-insoluble products in 30 minutes at 74℃ with activated salmon sperm DNA as the template-primer.

purity
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Nicking, endonuclease and exonuclease activity were not detected after the incubation of 0.6 μg of supercoiled pBR322 DNA, 0.6 μg of λDNA or 0.6 μg of λ-Hin d III digest with 10 units of this enzyme for 1 hour at 74℃.

Source
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Escherichia coli carrying a plasmid that encodes the Thermus aquaticus DNA Polymerase gene.

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Takara Bio companies provide kits, reagents, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, DSS Takara Bio India Private Ltd. is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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