Takara Taq (r Taq DNA Polymerase without dNTPs)
Takara Taq DNA Polymerase is a recombinant version of full-length Taq polymerase. It has the same characteristics and capabilities as the native Taq polymerase, and is suitable for a variety of standard PCR applications. The polymerase is supplied with separate tube of buffer (Mg2+ plus).
Product | Catalogue No. | Size |
| Takara Taq DNA Polymerase | R500A | 250 Units |
-20℃
1. For DNA amplification by PCR-well mini-gel, respectively.
2. For DNA sequencing
| Tris-HCl (pH8.0) | 20 mM |
| KCI | 100 mM |
| EDTA | 0.1 mM |
| 0.2 mM DTT | 1 mM |
| Tween 20 | 0.5% |
| Nonidet P-40 | 0.5% |
| Glycerol | 50% |
One unit is the amount of enzyme that will incorporate10 nmol of dNTP into acid-insoluble products in 30 minutes at 74℃ with activated salmon sperm DNA as the template-primer.
Nicking, endonuclease and exonuclease activity were not detected after the incubation of 0.6 μg of supercoiled pBR322 DNA, 0.6 μg of λDNA or 0.6 μg of λ-Hin d III digest with 10 units of this enzyme for 1 hour at 74℃.
Escherichia coli carrying a plasmid that encodes the Thermus aquaticus DNA Polymerase gene.
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