Takara Taq™ Hot Start Version
The hot-start versions of Takara Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody that binds to Taq polymerase, thereby preventing DNA synthesis at room temperature. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot-start PCR enzyme prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during PCR assembly.
Note: Please enquire for the protocol of the Glycerol Free ranges.
• Takara Taq DNA Polymerase Hot Start Version—enzyme, buffer, and dNTPs are supplied as separate components
• Premix Taq DNA Polymerase Hot Start Version—2X premix containing Takara Taq HS enzyme, dNTPs, and an optimized reaction buffer
• Takara Taq HS Perfect Mix—2X hot-start premix optimized for fast reactions. It contains a modified Taq DNA polymerase, which lacks exonuclease activities, and is compatible with a 20 sec/kb extension step
| Hot-start PCR |
| Multiplex PCR |
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Takara Bio companies provide kits, reagents, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, DSS Takara Bio India Private Ltd. is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.
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