RNAiso Blood is a total RNA extraction reagent that can easily and quickly isolate RNA from human or animal blood samples or samples containing a large amount of water.

After adding RNAiso Blood solution to disrupt tissues or cells, add chloroform to the disrupted solution, mix well, and centrifuge to separate into three layers. At this time, RNA is in the upper transparent water layer, DNA is in the semi-solid middle layer, and finally, proteins, polysaccharides, fatty acids, cell debris, and a small amount of DNA are in the lower organic solvent layer that is red *

Here, only the upper transparent water layer can be collected and total RNA can be recovered by isopropanol precipitation. Using this product, the entire total RNA extraction process can be completed in about 1 hour, and the separated total RNA can be used for RT-PCR **, Northern blot analysis, mRNA separation, and in vitro translation reactions.

* Since the organic solvent layer in this product is red, it is easy to distinguish the boundary, making it more convenient to use.

** When using purified RNA samples for RT-PCR, since even a small amount of gDNA contamination can significantly affect the results, we recommend treatment with Recombinant DNase I (RNase-free) (Code 2270A) before use.