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DTI Green HiFid Taq HS Premix
Brand | Catalogue Number | Pack Size |
DTI Green HiFid Taq HS Premix | DT0602.100 | 100 rxn (20 μl volume) |
DTI Green HiFid Taq HS Premix | DT0602.500 | 500 rxn (20 μl volume) |
• Use with broad range of amplicon sizes, even over 500 bp
• Convenient 2X master mix is excellent for high-speed qPCR
• High sensitivity: detects fewer than 100 copies
• Hot-start PCR enzyme enables high efficiency and sensitivity in real-time PCR (qPCR)
• Compatible with all commonly used real-time PCR (qPCR) instruments
• Minimizes qPCR inhibition due to residual mRNA in input cDNA
• Includes DTI Green for intercalator-based qPCR
Results
qPCR without optimization using DTI Green HiFid Taq HS Premix
Conclusions
Methods
Component |
DTI Green HiFid Taq HS Premix (Tli RNaseH Plus) (2X) |
Forward Primer (10 µM) |
Reverse Primer (10 µM) |
ROX Reference Dye (50X) |
Template |
Sterile dH2O |
Total |
Amount |
10 µl |
0.4 µl |
0.4 µl |
0.4 µl |
2.0 µl |
6.8 µl |
20 µl |
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