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DTI FabTaq DNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticus YT-1 strain, and is suitable for routine PCR applications.

For individual reaction setup and optimization, use individual components of enzyme, dNTP mixture, and 10X reaction buffer (with or without Mg2+).

Specifications

Documents

Applications

FAQs

Brand	Catalogue Number	Pack Size
DTI FabTaq	DT0101.250	250 U
DTI FabTaq	DT0101.500	500 U
DTI FabTaq	DT0101.1K	1000 U

• Excellent for standard PCR amplifications.

• Low bacterial DNA contamination.

• Premix Taq enables easy PCR setup and minimizes pipetting steps.

DTI FabTaq - Protocol

DTI FabTaq - Certificate of Analysis

DTI FabTaq - Promotional Document

Results

Amplification consistency of 8 kb DNA fragment

Consistent amplification of 8 kb fragment from λ DNA is observed using the DTI FabTaq. The

protocol used for the assay and the results are as follows:

Which enzymes faciliate efficient workflow when analyzing multiple samples by gel electrophoresis after PCR (e.g., genotyping screens)?

We recommend DTI JadeAmp FabTaq Premix (Cat.# DT0201.320). This product is completely premixed, making it easy to prepare PCR reaction mixtures which can be loaded directly on an agarose gel for electrophoresis after the reaction. For an efficient workflow with minimal pipetting, DTI JadeAmp FabTaq Premix contains a green tracking dye. Additionally, density agent is included to aid in gel loading.

DTI JadeAmp FabTaq Premix can amplify targets up to 10 kb in length, including targets that are GC- or AT-rich. Additionally, PCR products generated with DTI JadeAmp FabTaq Premix can be used directly for restriction enzyme digestion, sequencing, or TA-cloning without need for further purification.

Which enzymes are suitable for colony PCR?

We recommend DTI CobaltAmp HiFid Taq HS Premix for colony PCR. These enzyme preparations tolerate the presence of substantial bacterial nucleic acid carry-over. Because tracking dye and density agent are included in these master mixes, the PCR reaction mixtures may be loaded directly on an agarose gel for electrophoresis.

Which enzymes are suitable for fast PCR?

DTI CobaltAmp HiFid Taq HS Premix is an economical choice for high-throughput projects. This enzyme is formulated to include high speed polymerase, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent in a 2X premix. Since it requires an extension time of only 10 sec/kb, colony PCR reactions can be completed in less than 1 hour for inserts up to 1 kb.

What precautions should be taken when using an inosine-containing primer?

DTI FabTaq and DTI FabTaq Hot Start Version enzymes are compatible with inosine-containing primers. It is important to note that inosine-containing primers should not be used with PCR enzymes that have 3’→5’ exonuclease activity. When using one of these PCR enzymes, we recommend using mixtures of degenerate primers with A, T, G, and C at the desired position(s) rather than inosine-containing primers when performing degenerate PCR.

What is the terminal structure of DTI FabTaq and DTI FabTaq Hot Start Version enzymes’ amplification product?

DTI FabTaq and DTI FabTaq Hot Start Version enzymes primarily yield amplification products containing 3’-dA overhangs, which facilitates TA cloning.

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