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The DTI FabSpin Blood DNA Kit II is designed to purify genomic DNA from whole blood. This kit employs a proprietary lysis buffer in combination with Spin Column membrane to efficiently purify genomic DNA from the biological sample. The protocol provides a simple method to achieve the rapid isolation of highly purified genomic DNA and the entire procedure can be accomplished within 30 mins. Genomic DNA obtained is suitable or a variety of applications, such as PCR, Southern blotting, RAPD, AFLP, RFLP and other molecular experiments.

Specifications

Documents

Applications

Brand	Catalogue Number	Pack Size
DTI FabSpin Blood DNA Kit II	DT9702.100P	100 Preps
DTI FabSpin Blood DNA Kit II	DT9702.250P	250 Preps

➥ Kit Specifications:

• The DTI FabSpin Blood DNA Kit II is designed for rapid isolation of high purity DNA from whole blood of higher

mammals including humans with non-nucleated Red blood cells RBCs

o This kit can also be used for extraction of genomic DNA from birds and fish containing nucleated RBCs

• DNA can be purified successfully from blood samples collected in EDTA, ACD or heparin anti-coagulant. DNA can

also be purified from buffy coat of human blood sample.

• High pure genomic DNA with an A260/A280 ratio between 1.8-2.0 with a concentration of 3-5 µg from healthy

individual can be obtained using this kit.

• Genomic DNA obtained is suitable for a variety of applications, such as PCR, Southern

blotting, RAPD, AFLP, RFLP and other molecular experiments.

➥ Storage and Shipment Conditions:

• The buffer components are to be stored at room temperature (15 - 25°C).

• Once received, store the Lyophilised powder preparations of Proteinase K at -20°C

• Shipment temperature of the kit is room temperature (15 - 25°C).

➥ Components:

Components	Cat# DT9701.100P (100 Preps)	Cat# DT9701.250P (250 Preps)
Proteinase K (Lyophilised)	40 mg	100 mg
Proteinase K Reconstitution Buffer	2 ml	5 ml
Buffer GB **	24 ml	60 ml
Buffer WA **	56 ml	140 ml
Buffer WB	48 ml	120 ml
Elution Buffer	28 ml	70 ml
PBS	20 ml	50 ml
Nuclease Free Water	5 ml	12.5 ml
Spin Columns (Green Ring)	100 pcs	250 pcs
Collection Tubes	100 pcs	250 pcs
SDS Insert Sheet	1	1
CoA	1	1

* Sample Pack, available only on request

**Contains strong denaturant. Avoid contact with skin and eyes. In case of such contact, wash immediately with plenty of water and seek medical advice. Please refer the safety data sheet

➥ Materials required but not provided:

• 100% ethanol

• RNAse A (10 mg / mL) (as required)

• Centrifuge

• Vortex

• Water bath (56°C)

• Micropipettes (2-200 µl, 10-1000 µl)

• Pipette tips (200 µl, 1000 µl)

• Personnel protection equipment including labcoat, gloves and goggles

• Proteinase-K to be purchased separately for catalogue numbers DT9702.25/ DT9702.100/ DT9702.250

➥ Preparation Before Experiment:

i. Proteinase K preparation:

For Cat# DT9702.25P, dissolve 10 mg of lyophilised Proteinase K in 500 µl of reconstitution buffer provided in the kit.

For Cat# DT9702.100P, dissolve 40 mg of lyophilised Proteinase K in 2 ml of reconstitution buffer provided in the kit

For Cat# DT9702.250P, dissolve 100 mg of lyophilised Proteinase K in 5 ml of reconstitution buffer provided in the kit.

ii. Adjust a water bath to 56°C.

iii. Before using the kit:

For Cat# DT9702.25P, add 28 ml of 100% ethanol to Buffer WB (12 ml). Mix well.

For Cat# DT9702.100P, add 112 ml of 100% ethanol to Buffer WB (48 ml). Mix well.

For Cat# DT9702.250P, add 280 ml of 100% ethanol to Buffer WB (120 ml). Mix well.

iv. Pre-heat the Elution Buffer/nuclease free water to 65°C to improve elution efficiency.

DTI FabSpin Blood DNA Kit II - Protocol

DTI FabSpin Blood DNA Kit II - Certificate of Analysis

DTI FabSpin Blood DNA Kit II - Promotional Document

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