Home > Products > PCR > DTI Bca DNA Polymerase - Glycerol Free

DTI Bca DNA Polymerase- Glycerol Free originates from a thermophilic bacterium Bacillus caldotenax, that lacks 5’ → 3’ exonuclease activity. By introducing mutations into the protein, the activity of both DNA polymerase and reverse transcriptase is highly enhanced while exerting the original strong strand-displacement activity. Therefore, the isothermal amplifications using this enzyme enable to amplify the target product from a little nucleic acid in a shorter time. This enzyme is originally isolated and developed independently by Takara Bio Inc, Japan. DTI manufactures and sells it under an agreement with Takara Bio Inc.

Specifications

Documents

Applications

Brand	Catalogue Number	Pack Size
DTI Bca DNA Polymerase- Glycerol Free (8U/μl)	DT0104.80	80 U

➥ Overview:

• Improved sensitivity compared to BcaBEST DNA Polymerase

• Reaction completes in 30 min or less

• High thermostability

• Compatible with real-time fluorescent monitoring and colorimetric/visual detection methods

➥ Storage and Shipment Conditions:

-80°C

➥ Components:

Components	Pack Size
DTI Bca DNA Polymerase- Glycerol Free (8U/μl)	10 µl

* Customized pack size available

➥ Materials required but not provided:

• 2X Bca BEST buffer (Component of Takara Cat# RR380A)

• 10X target specific LAMP primer mix

• Template DNA/cDNA

• For RT-LAMP, Reverse Transcriptase

• Nuclease free water

• For real time LAMP assay: Inter-calating dye, Thermal cycler

➥ Source:

Escherichia coli carrying a plasmid containing the gene for BCA DNA Polymerase

➥ Unit definition:

One unit is defined as the amount of enzyme that incorporates 210 nmol of dNTP into acid-insoluble precipitate in 30 minute at 65°C.

➥ Application:

1. LAMP (Loop-Mediated Isothermal Amplification)

2. RCA (Rolling Circle Amplification)

➥ Precautions for use:

1. Don’t mix the enzyme vigorously.

2. Extreme cautions should be taken during reaction preparation to avoid contamination. If analysing the products

after reaction, execute in a different area from the reaction preparation.

3. Reaction temperatures above 70℃ are not recommended. Hence this enzyme can’t be used for conventional PCR.

DTI Bca DNA Polymerase - Glycerol Free - Protocol

DTI Bca DNA Polymerase - Glycerol Free - Certificate of Analysis

DTI Bca DNA Polymerase - Glycerol Free - Promotional Document

Experimental Sample

1. LAMP assay: Target gene was detected using 0.01 – 0.01 ng of E. coli genomic DNA when the isothermal amplification was performed using the enzyme. The protocol used was : Incubate at 63℃ for 90 minutes

2. ii) RT-LAMP assay: Target gene was detected using 0.01 – 0.01 ng of human total RNA when the RT- isothermal amplification was performed using AMV reverse transcriptase (Takara cat# 2630A) and Bca DNA polymerase. The protocol used was: Incubate at 63℃ for 90 minutes

Get-In-Touch With Us

Takara Bio companies provide kits, reagents, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, DSS Takara Bio India Private Ltd. is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.