DTI FabSpin Blood DNA Kit II
DTI FabSpin Blood DNA Kit II
The DTI FabSpin Blood DNA Kit II is designed to purify genomic DNA from whole blood. This kit employs a proprietary lysis buffer in combination with Spin Column membrane to efficiently purify genomic DNA from the biological sample. The protocol provides a simple method to achieve the rapid isolation of highly purified genomic DNA and the entire procedure can be accomplished within 30 mins. Genomic DNA obtained is suitable or a variety of applications, such as PCR, Southern blotting, RAPD, AFLP, RFLP and other molecular experiments.
SKU:DT9702.100P
DTI FabSpin Blood DNA Kit II
| Brand | Catalogue Number | Pack Size |
|---|---|---|
| DTI FabSpin Blood DNA Kit II | DT9702.100P | 100 Preps |
| DTI FabSpin Blood DNA Kit II | DT9702.250P | 250 Preps |
Product Description: The DTI FabSpin Blood DNA Kit II is designed for rapid isolation of high purity DNA from whole blood of higher mammals, including humans with non-nucleated Red blood cells (RBCs). It can also be used for genomic DNA extraction from birds and fish containing nucleated RBCs.
Kit Specifications:
- High purity genomic DNA: Obtained with an A260/A280 ratio between 1.8-2.0 and concentration of 3-5 µg from healthy individuals.
- Suitable for various applications: PCR, Southern blotting, RAPD, AFLP, RFLP, and more molecular experiments.
- Compatible with EDTA, ACD, or heparin anti-coagulant blood samples: DNA purification can be done from buffy coat of human blood sample.
Storage and Shipment Conditions:
- Buffer components: Store at room temperature (15 - 25°C).
- Proteinase K (Lyophilised): Store at -20°C after receipt.
- Shipment temperature: Room temperature (15 - 25°C).
Components:
| Components | Cat# DT9701.100P (100 Preps) | Cat# DT9701.250P (250 Preps) |
|---|---|---|
| Proteinase K (Lyophilised) | 40 mg | 100 mg |
| Proteinase K Reconstitution Buffer | 2 ml | 5 ml |
| Buffer GB | 24 ml | 60 ml |
| Buffer WA | 56 ml | 140 ml |
| Buffer WB | 48 ml | 120 ml |
| Elution Buffer | 28 ml | 70 ml |
| PBS | 20 ml | 50 ml |
| Nuclease Free Water | 5 ml | 12.5 ml |
| Spin Columns (Green Ring) | 100 pcs | 250 pcs |
| Collection Tubes | 100 pcs | 250 pcs |
| SDS Insert Sheet | 1 | 1 |
| CoA | 1 | 1 |
| * Sample Pack | Available on request | Available on request |
Materials required but not provided:
- 100% ethanol
- RNAse A (10 mg / mL) (as required)
- Centrifuge
- Vortex
- Water bath (56°C)
- Micropipettes (2-200 µl, 10-1000 µl)
- Pipette tips (200 µl, 1000 µl)
- Personnel protection equipment including labcoat, gloves, and goggles
- Proteinase-K to be purchased separately for catalogue numbers DT9702.25/ DT9702.100/ DT9702.250
Preparation Before Experiment:
- Proteinase K preparation: Dissolve the appropriate amount of lyophilised Proteinase K in the provided reconstitution buffer.
- Adjust water bath to 56°C.
- Before using the kit: Add the specified amount of 100% ethanol to Buffer WB and mix well.
- Pre-heat Elution Buffer/nuclease-free water to 65°C for improved elution efficiency.
DTI FabSpin Blood DNA Kit II - Protocol and Downloads
| Document | Download Link |
|---|---|
| DTI FabSpin Blood DNA Kit II - Protocol | Download User Manual |
| DTI FabSpin Blood DNA Kit II - Certificate of Analysis | Download CoA - DT9702.25P |
| DTI FabSpin Blood DNA Kit II - Certificate of Analysis | Download CoA - DT9702.100P |
| DTI FabSpin Blood DNA Kit II - Certificate of Analysis | Download CoA - DT9702.250P |
| DTI FabSpin Blood DNA Kit II - Promotional Document | Download Flyer |